8ZAE

Crystal structure of RuABA3 from Rutstroemia sp. NJR-2017a WRK4 in complex with FsPP

Summary for 8ZAE

• Entry DOI: 10.2210/pdb8zae/pdb
• Descriptor: Aba 3 protein, S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL] TRIHYDROGEN THIODIPHOSPHATE, ZINC ION, ... (5 entities in total)
• Functional Keywords: ruaba3, sesquiterpene synthases, fspp, lyase
• Biological source: Rutstroemia sp. NJR-2017a WRK4
• Total number of polymer chains: 2
• Total formula weight: 94493.92

Authors

Li, S.Y.,Li, H.,Yang, Y.,Huang, J.-W.,Chen, C.-C.,Guo, R.-T. (deposition date: 2024-04-25, release date: 2024-12-11, Last modification date: 2025-01-22)

Primary citation

Li, S.,Huang, J.W.,Min, J.,Li, H.,Ning, M.,Zhou, S.,Yang, Y.,Chen, C.C.,Guo, R.T.
Molecular insights into a distinct class of terpenoid cyclases.
Nat Commun, 16:207-207, 2025

PubMed Abstract: Terpenoid cyclases (TCs) account for the synthesis of the most widespread and diverse natural compounds. A sesquiterpene cyclase termed BcABA3 from an abscisic acid-producing fungus Botrytis cinerea that yields (2Z,4E)-α-ionylideneethane but lacks signature feature of canonical TCs represents a distinct type of TCs. Here, we report the crystal structures of BcABA3, a closely related RuABA3 from Rutstroemia sp. and a bacterial SkABA3 from Shimazuella kribbensis. These ABA3 proteins adopt an all-α-helix fold and bind pyrophosphate moiety of farnesyl pyrophosphate by Glu-chelated Mg ion cluster. We conduct mutagenesis experiments to validate the role of the substrate-binding residues. SkABA3 appears to yield compounds that are distinct from (2Z,4E)-α-ionylideneethane. These results not only provide the molecular insight into ABA3 proteins that serve as an important basis to the future investigation of this class of TCs, but also reveal the existence of more uncharacterized terpenoids synthesized via dedicated machineries.

PubMed: 39747870
DOI: 10.1038/s41467-024-55717-6

Experimental method

X-RAY DIFFRACTION (2.28 Å)