8Z9A

Cryo-EM structure of the insect olfactory receptor OR5-Orco heterocomplex from Acyrthosiphon pisum bound with geranyl acetate

Summary for 8Z9A

• Entry DOI: 10.2210/pdb8z9a/pdb
• EMDB information: 39858
• Descriptor: Odorant receptor, ApisOrco, Odorant receptor, ApisOR5, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total)
• Functional Keywords: membrane protein
• Biological source: Acyrthosiphon pisum (pea aphid); More
• Total number of polymer chains: 4
• Total formula weight: 206931.67

Authors

Wang, Y.D.,Qiu, L.,Guan, Z.Y.,Wang, Q.,Wang, G.R.,Yin, P. (deposition date: 2024-04-23, release date: 2024-06-19, Last modification date: 2024-07-10)

Primary citation

Wang, Y.,Qiu, L.,Wang, B.,Guan, Z.,Dong, Z.,Zhang, J.,Cao, S.,Yang, L.,Wang, B.,Gong, Z.,Zhang, L.,Ma, W.,Liu, Z.,Zhang, D.,Wang, G.,Yin, P.
Structural basis for odorant recognition of the insect odorant receptor OR-Orco heterocomplex.
Science, 384:1453-1460, 2024

PubMed Abstract: Insects detect and discriminate a diverse array of chemicals using odorant receptors (ORs), which are ligand-gated ion channels comprising a divergent odorant-sensing OR and a conserved odorant receptor co-receptor (Orco). In this work, we report structures of the OR5-Orco heterocomplex from the pea aphid alone and bound to its known activating ligand, geranyl acetate. In these structures, three Orco subunits serve as scaffold components that cannot bind the ligand and remain relatively unchanged. Upon ligand binding, the pore-forming helix S7b of OR5 shifts outward from the central pore axis, causing an asymmetrical pore opening for ion influx. Our study provides insights into odorant recognition and channel gating of the OR-Orco heterocomplex and offers structural resources to support development of innovative insecticides and repellents for pest control.

PubMed: 38870272
DOI: 10.1126/science.adn6881

Experimental method

ELECTRON MICROSCOPY (3 Å)