8YXI

Crystal structure of SFTSV Gn in complex with a neutralizing antibody 40C10

8YXI の概要

• エントリーDOI: 10.2210/pdb8yxi/pdb
• 分子名称: heavy chain, light chain, Envelopment polyprotein, ... (6 entities in total)
• 機能のキーワード: neutralizing antibody, sftsv, complex, viral protein/immune system, viral protein-immune system complex
• 由来する生物種: Severe fever with thrombocytopenia syndrome virus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 86847.85

構造登録者

Yang, P.,Guo, Y.,Zhang, N. (登録日: 2024-04-02, 公開日: 2024-09-11, 最終更新日: 2025-01-22)

主引用文献

Yang, P.,Wu, X.,Shang, H.,Sun, Z.,Wang, Z.,Song, Z.,Yuan, H.,Deng, F.,Shen, S.,Guo, Y.,Zhang, N.
Molecular mechanism and structure-guided humanization of a broadly neutralizing antibody against SFTSV.
Plos Pathog., 20:e1012550-e1012550, 2024

PubMed Abstract: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a novel tick-borne bunyavirus that causes severe fever with thrombocytopenia syndrome (SFTS), with a high mortality rate of up to 30%. The envelope glycoproteins of SFTSV, glycoprotein N (Gn) and glycoprotein C (Gc), facilitate the recognition of host receptors and the process of membrane fusion, allowing the virus to enter host cells. We previously reported a monoclonal antibody, mAb 40C10, capable of neutralizing different genotypes of SFTSV and SFTSV-related viruses. However, the specific neutralization mechanism is poorly understood. In this study, we elucidated the high-resolution structure of the SFTSV Gn head domain in complex with mAb 40C10, confirming that the binding epitope in the domain I region of SFTSV Gn, and it represented that a novel binding epitope of SFTSV Gn was identified. Through in-depth structural and sequence analyses, we found that the binding sites of mAb 40C10 are relatively conserved among different genotypes of SFTSV and SFTSV-related Heartland virus and Guertu virus, elucidating the molecular mechanism underlying the broad-spectrum neutralizing activity of mAb 40C10. Furthermore, we humanized of mAb 40C10, which is originally of murine origin, to reduce its immunogenicity. The resulting nine humanized antibodies maintained potent affinity and neutralizing activity. One of the humanized antibodies exhibited neutralizing activity at picomolar IC50 values and demonstrated effective therapeutic and protective effects in a mouse infection model. These findings provide a novel target for the future development of SFTSV vaccines or drugs and establish a foundation for the research and development of antibody therapeutics for clinical applications.

PubMed: 39321193
DOI: 10.1371/journal.ppat.1012550

実験手法

X-RAY DIFFRACTION (2.4 Å)