8YRL
Crystal structure of Aspergillus fumigatus Galactofuranosylransferase (AfGfsA) in complex with UDP and galactofuranose
Summary for 8YRL
| Entry DOI | 10.2210/pdb8yrl/pdb |
| Descriptor | Glycosyltransferase family 31 protein, MANGANESE (II) ION, URIDINE-5'-DIPHOSPHATE, ... (6 entities in total) |
| Functional Keywords | galactofuranosylransferase galactofuranose, transferase |
| Biological source | Aspergillus fumigatus CEA10 |
| Total number of polymer chains | 2 |
| Total formula weight | 114957.99 |
| Authors | Okuno, A.,Oka, T.,Chihara, Y.,Hirata, R.,Kadooka, C.,Teramoto, T.,Hira, D.,Kakuta, Y. (deposition date: 2024-03-21, release date: 2024-10-30, Last modification date: 2024-11-20) |
| Primary citation | Oka, T.,Okuno, A.,Hira, D.,Teramoto, T.,Chihara, Y.,Hirata, R.,Kadooka, C.,Kakuta, Y. Substrate binding and catalytic mechanism of UDP-alpha-D-galactofuranose: beta-galactofuranoside beta-(1→5)-galactofuranosyltransferase GfsA. Pnas Nexus, 3:pgae482-pgae482, 2024 Cited by PubMed Abstract: UDP-α-D-galactofuranose (UDP-Galf): β-galactofuranoside β-(1→5)-galactofuranosyltransferase, known as GfsA, is essential in synthesizing β-(1→5)-galactofuranosyl oligosaccharides that are incorporated into the cell wall of pathogenic fungi. This study analyzed the structure and function of GfsA from . To provide crucial insights into the catalytic mechanism and substrate recognition, the complex structure was elucidated with manganese (Mn), a donor substrate product (UDP), and an acceptor sugar molecule (β-galactofuranose). In addition to the typical GT-A fold domain, GfsA has a unique domain formed by the N and C termini. The former interacts with the GT-A of another GfsA, forming a dimer. The active center that contains Mn, UDP, and galactofuranose forms a groove structure that is highly conserved in the GfsA of Pezizomycotina fungi. Enzymatic assays using site-directed mutants were conducted to determine the roles of specific active-site residues in the enzymatic activity of GfsA. The predicted enzyme-substrate complex model containing UDP-Galf characterized a specific β-galactofuranosyltransfer mechanism to the 5'-OH of β-galactofuranose. Overall, the structure of GfsA in pathogenic fungi provides insights into the complex glycan biosynthetic processes of fungal pathogenesis and may inform the development of novel antifungal therapies. PubMed: 39507050DOI: 10.1093/pnasnexus/pgae482 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.37 Å) |
Structure validation
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