8YR0

Cryo-EM Structure of AdeG from Acinetobacter baumannii

Summary for 8YR0

• Entry DOI: 10.2210/pdb8yr0/pdb
• EMDB information: 39532
• Descriptor: Cation/multidrug efflux pump, DODECYL-BETA-D-MALTOSIDE (2 entities in total)
• Functional Keywords: efflux pump, acinetobacter baumannii, multidrug resistance, adeg, membrane protein
• Biological source: Acinetobacter baumannii
• Total number of polymer chains: 3
• Total formula weight: 338944.77

Authors

Ouyang, Z.,Wen, Y.,Zhu, L. (deposition date: 2024-03-20, release date: 2025-01-01, Last modification date: 2026-07-15)

Primary citation

Ouyang, Z.,He, W.,Wu, D.,An, H.,Duan, L.,Jiao, M.,He, X.,Yu, Q.,Zhang, J.,Qin, Q.,Wang, R.,Zheng, F.,Hwang, P.M.,Hua, X.,Zhu, L.,Wen, Y.
Cryo-EM structure and complementary drug efflux activity of the Acinetobacter baumannii multidrug efflux pump AdeG.
Structure, 33:539-551.e4, 2025

PubMed Abstract: Multidrug-resistant Acinetobacter baumannii has emerged as one of the most antibiotic-resistant bacterial pathogens associated with nosocomial infection, with its resistance highly depending on multiple multidrug efflux pumps. Here, we report the cryoelectron microscopy (cryo-EM) structure of Acinetobacter drug efflux G (AdeG), the inner membrane component of one of three important resistance-nodulation-cell division (RND) pump family members in A. baumannii, which is involved in drug resistance to chloramphenicol, trimethoprim, ciprofloxacin, and clindamycin. We systematically compare the structures and substrate binding specificities of AdeG, AdeB, and AdeJ multidrug efflux pumps via molecular docking, revealing potential determinants for drug binding. Knockout experiments demonstrate a functional complementarity between AdeABC, AdeFGH, and AdeIJK. Our study provides a structural understanding of A. baumannii multidrug efflux pump AdeG and reveals complementary drug efflux activity between AdeG and other RND efflux pumps, which may promote further rational drug discovery efforts targeting multidrug efflux pumps.

PubMed: 39798571
DOI: 10.1016/j.str.2024.12.009

Experimental method

ELECTRON MICROSCOPY (3.06 Å)