8YE8

Crystal structure of mouse BAHCC1 TTD domain in complex with H4K20me1 peptide

Summary for 8YE8

• Entry DOI: 10.2210/pdb8ye8/pdb
• Descriptor: BAH and coiled-coil domain-containing protein 1, Histone H4 (3 entities in total)
• Functional Keywords: bahcc1, h4k20me, protein binding
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 3
• Total formula weight: 32964.04

Authors

Zhang, Z.-M.,Song, J. (deposition date: 2024-02-22, release date: 2025-07-16)

Primary citation

Li, D.,Zhang, Z.M.,Mei, L.,Yu, Y.,Guo, Y.,Mackintosh, S.G.,Chen, J.,Allison, D.F.,Kim, A.,Storey, A.J.,Edmondson, R.D.,Byrum, S.D.,Tackett, A.J.,Cai, L.,Cook, J.G.,Song, J.,Wang, G.G.
BAHCC1 binds H4K20me1 to facilitate the MCM complex loading and DNA replication.
Nat Commun, 16:5502-5502, 2025

PubMed Abstract: Mono-methylation of histone H4 lysine 20 (H4K20me1) regulates DNA replication, cell cycle progression and DNA damage repair. How exactly H4K20me1 regulates these biological processes remains unclear. Here, we report that an evolutionarily conserved tandem Tudor domain (TTD) in BAHCC1 (BAHCC1) selectively reads H4K20me1 for facilitating replication origin activation and DNA replication. Our biochemical, structural, genomic and cellular analyses demonstrate that BAHCC1 preferentially recognizes H4K20me1 to promote the recruitment of BAHCC1 and its interacting partners, notably Mini-chromosome Maintenance (MCM) complex, to replication origin sites. Combined actions of the H4K20me1-reading BAHCC1 and the H4K20me2-reading Origin Recognition Complex (ORC) ensure genomic loading of MCM for replication. Depletion of BAHCC1, or disruption of the BAHCC1:H4K20me1 interaction, reduces H4K20me1 levels and MCM loading, leading to defects in replication origin activation and cell cycle progression. In summary, this study identifies BAHCC1 as an effector transducing H4K20me1 signals into MCM recruitment to promote DNA replication.

PubMed: 40592879
DOI: 10.1038/s41467-025-61284-1

Experimental method

X-RAY DIFFRACTION (1.90001382876 Å)