8YDM
Cryo-EM structure of CaRC-LH complex from Chloroflexus aurantiacus
Summary for 8YDM
| Entry DOI | 10.2210/pdb8ydm/pdb |
| EMDB information | 39177 |
| Descriptor | Light-harvesting protein B-808/866 alpha chain, (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE, 2-methyl-3-[(2E,6E,10E,14E,18E,22E,26E,30E,34E,38E)-3,7,11,15,19,23,27,31,35,39,43-undecamethyltetratetraconta-2,6,10,1 4,18,22,26,30,34,38,42-undecaen-1-yl]naphthalene-1,4-dione, ... (13 entities in total) |
| Functional Keywords | photosynthesis |
| Biological source | Chloroflexus aurantiacus J-10-fl More |
| Total number of polymer chains | 18 |
| Total formula weight | 246994.76 |
| Authors | Guoqiang, H.,Shishang, D. (deposition date: 2024-02-20, release date: 2025-02-19, Last modification date: 2025-05-07) |
| Primary citation | Huang, G.,Dong, S.,Ma, L.,Li, L.,Ju, J.,Wang, M.J.,Zhang, J.P.,Sui, S.F.,Qin, X. Cryo-EM structure of a minimal reaction center-light-harvesting complex from the phototrophic bacterium Chloroflexus aurantiacus. J Integr Plant Biol, 67:967-978, 2025 Cited by PubMed Abstract: Photosynthetic organisms have developed various light-harvesting antenna systems to capture light and transfer energy to reaction centers (RCs). Simultaneous utilization of the integral membrane light-harvesting antenna (LH complex) and the extrinsic antenna (chlorosomes) makes the phototrophic bacterium Chloroflexus (Cfx.) aurantiacus an ideal model for studying filamentous anoxygenic phototrophs (FAPs). Here, we determined the structure of a minimal RC-LH photocomplex from Cfx. aurantiacus J-10-fl (CaRC-LH) at 3.05-Å resolution. The CaRC-LH binds only to seven LH subunits, which form a crescent-shaped antenna surrounding the movable menaquinone-10 (Q) binding site of CaRC. In this complex with minimal LH units, an extra antenna is required to ensure sufficient light-gathering, providing a clear explanation for the presence of chlorosomes in Cfx. aurantiacus. More importantly, the semicircle of the antenna represents a novel RC-LH assembly pattern. Our structure provides a basis for understanding the existence of chlorosomes in Cfx. aurantiacus and the possible assembly pattern of RC-LH. PubMed: 39912559DOI: 10.1111/jipb.13853 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.05 Å) |
Structure validation
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