8Y9Q

b-glucosidase from Thermotoga profunda Tp-BGL

Summary for 8Y9Q

• Entry DOI: 10.2210/pdb8y9q/pdb
• Descriptor: b-glucosidase, GLYCEROL (3 entities in total)
• Functional Keywords: glycoside hydrolase, cellulase, glucosidase, hydrolase
• Biological source: Thermotoga profunda
• Total number of polymer chains: 2
• Total formula weight: 104419.98

Authors

Guo, Y.,Chen, A. (deposition date: 2024-02-07, release date: 2025-02-12)

Primary citation

Guo, Y.,Chen, A.,Liu, K.,Ji, C.
Structural insights and functional characterization of a novel beta-glucosidase derived from Thermotoga profunda.
Biochem.Biophys.Res.Commun., 732:150405-150405, 2024

PubMed Abstract: β-Glucosidase is a crucial cellulase, as its activity determines the efficiency of cellulose hydrolysis into glucose. This study addresses the functional and structural characteristics of Thermotoga profunda β-glucosidase (Tp-BGL). Tp-BGL exhibited a K of 0.3798 mM for p-nitrophenyl-β-d-glucopyranoside (pNPGlc) and 4.44 mM for cellobiose, with k/K of 1211.16 and 4.18 s mM, respectively. In addition, Tp-BGL showed significant pH adaptability and thermal stability, with a T of 85.7 °C and retaining >90 % of its activity after incubation at 80 °C for 90 min. The crystal structure of Tp-BGL was resolved at 1.95 Å resolution, and reveals a typical TIM barrel structure. Comparative structural analysis highlighted that the major distinction between Tp-BGL and the other glucosidases lies in their loop regions.

PubMed: 39033552
DOI: 10.1016/j.bbrc.2024.150405

Experimental method

X-RAY DIFFRACTION (1.96 Å)