8Y6A

Cryo-EM structure of SARS-CoV-2 Omicron BA.2.86 RBD in complex with human ACE2 and S309 Fab

Summary for 8Y6A

• Entry DOI: 10.2210/pdb8y6a/pdb
• EMDB information: 38983
• Descriptor: Angiotensin-converting enzyme 2, Spike protein S1, heavy chain of S309 Fab, ... (9 entities in total)
• Functional Keywords: sars-cov-2, omicron, ba.2.86, rbd, s309, hace2, viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 146959.55

Authors

Li, L.J.,Gu, Y.H.,Qi, J.X.,Gao, G.F. (deposition date: 2024-02-02, release date: 2024-07-03, Last modification date: 2024-10-16)

Primary citation

Li, L.,Shi, K.,Gu, Y.,Xu, Z.,Shu, C.,Li, D.,Sun, J.,Cong, M.,Li, X.,Zhao, X.,Yu, G.,Hu, S.,Tan, H.,Qi, J.,Ma, X.,Liu, K.,Gao, G.F.
Spike structures, receptor binding, and immune escape of recently circulating SARS-CoV-2 Omicron BA.2.86, JN.1, EG.5, EG.5.1, and HV.1 sub-variants.
Structure, 32:1055-1067.e6, 2024

PubMed Abstract: The recently emerged BA.2.86, JN.1, EG.5, EG.5.1, and HV.1 variants have a growth advantage. In this study, we explore the structural bases of receptor binding and immune evasion for the Omicron BA.2.86, JN.1, EG.5, EG.5.1, and HV.1 sub-variants. Our findings reveal that BA.2.86 exhibits strong receptor binding, whereas its JN.1 sub-lineage displays a decreased binding affinity to human ACE2 (hACE2). Through complex structure analyses, we observed that the reversion of R493Q in BA.2.86 receptor binding domain (RBD) plays a facilitating role in receptor binding, while the L455S substitution in JN.1 RBD restores optimal affinity. Furthermore, the structure of monoclonal antibody (mAb) S309 complexed with BA.2.86 RBD highlights the importance of the K356T mutation, which brings a new N-glycosylation motif, altering the binding pattern of mAbs belonging to RBD-5 represented by S309. These findings emphasize the importance of closely monitoring BA.2.86 and its sub-lineages to prevent another wave of SARS-CoV-2 infections.

PubMed: 39013463
DOI: 10.1016/j.str.2024.06.012

Experimental method

ELECTRON MICROSCOPY (2.72 Å)