8XX4

ASFV RNAP elongation complex

Summary for 8XX4

• Entry DOI: 10.2210/pdb8xx4/pdb
• EMDB information: 38745
• Descriptor: DNA-directed RNA polymerase subunit, RNA polymerase subunit 6, DNA (5'-D(P*CP*GP*CP*AP*AP*CP*GP*GP*CP*GP*A)-3'), ... (13 entities in total)
• Functional Keywords: asfv, rnap, elongation complex, transcription/rna/dna, transcription-rna-dna complex
• Biological source: African swine fever virus (ASFV); More
• Total number of polymer chains: 11
• Total formula weight: 422746.28

Authors

Zhu, G.L.,Zhu, Y.,Zhu, Z.X.,Sun, F.,Zheng, H.X. (deposition date: 2024-01-17, release date: 2025-01-22)

Primary citation

Zhu, G.,Xi, F.,Zeng, W.,Zhao, Y.,Cao, W.,Liu, C.,Yang, F.,Ru, Y.,Xiao, S.,Zhang, S.,Liu, H.,Tian, H.,Yang, F.,Lu, B.,Sun, S.,Song, H.,Sun, B.,Zhao, X.,Tang, L.,Li, K.,He, J.,Guo, J.,Zhu, Y.,Zhu, Z.,Sun, F.,Zheng, H.
Structural basis of RNA polymerase complexes in African swine fever virus.
Nat Commun, 16:501-501, 2025

PubMed Abstract: African swine fever virus is highly contagious and causes a fatal infectious disease in pigs, resulting in a significant global impact on pork supply. The African swine fever virus RNA polymerase serves as a crucial multifunctional protein complex responsible for genome transcription and regulation. Therefore, it is essential to investigate its structural and functional characteristics for the prevention and control of African swine fever. Here, we determine the structures of endogenous African swine fever virus RNA polymerase in both nucleic acid-free and elongation states. The African swine fever virus RNA polymerase shares similarities with the core of typical RNA polymerases, but possesses a distinct subunit M1249L. Notably, the dynamic binding mode of M1249L with RNA polymerase, along with the C-terminal tail insertion of M1249L in the active center of DNA-RNA scaffold binding, suggests the potential of M1249L to regulate RNA polymerase activity within cells. These results are important for understanding the transcription cycle of the African swine fever virus and for developing antiviral strategies.

PubMed: 39779680
DOI: 10.1038/s41467-024-55683-z

Experimental method

ELECTRON MICROSCOPY (2.6 Å)