8XWB
Crystal structure of dinitrosyl iron units binding with human heavy chain Ferritin
Summary for 8XWB
| Entry DOI | 10.2210/pdb8xwb/pdb |
| Descriptor | Ferritin heavy chain, FE (III) ION, MAGNESIUM ION, ... (6 entities in total) |
| Functional Keywords | human ferritin, metal binding protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 20579.21 |
| Authors | |
| Primary citation | Gong, W.,Wu, T.,Liu, Y.,Jiao, S.,Wang, W.,Yan, W.,Li, Y.,Liu, Y.,Zhang, Y.,Wang, H. Insight into the photodynamic mechanism and protein binding of a nitrosyl iron-sulfur [Fe 2 S 2 (NO) 4 ] 2- cluster. Spectrochim Acta A Mol Biomol Spectrosc, 320:124603-124603, 2024 Cited by PubMed Abstract: Iron-sulfur cluster conversion and nitrosyl modification are involved in regulating their functions and play critical roles in signaling for biological systems. Hereby, the photo-induced dynamic process of (MeN)[FeS(NO)] was monitored using time-resolved electron paramagnetic resonance (EPR) spectra, MS spectra and cellular imaging methods. Photo-irradiation and the solvent affect the reaction rates and products. Spectroscopic and kinetic studies have shown that the process involves at least three intermediates: spin-trapped NO free radical species with a g at 2.040, and two other iron nitrosyl species, dinitrosyl iron units (DNICs) and mononitrosyl iron units (MNICs) with g values at 2.031 and 2.024, respectively. Moreover, the [FeS(NO)] cluster could bind with ferritin and decompose gradually, and a binding state of dinitrosyl iron coordinated with Cys102 of the recombinant human heavy chain ferritin (rHuHF) was finally formed. This study provides insight into the photodynamic mechanism of nitrosyl iron - sulfur clusters to improve the understanding of physiological activity. PubMed: 38878720DOI: 10.1016/j.saa.2024.124603 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.69 Å) |
Structure validation
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