8XPP

Crystal structure of the enterovirus 71 RdRP elongation complex with the nucleoside monophosphate form of compound HNC-1664 at product position -1 (post-translocation state)

これはPDB形式変換不可エントリーです。

8XPP の概要

• エントリーDOI: 10.2210/pdb8xpp/pdb
• 分子名称: Genome polyprotein, RNA (35-MER), RNA (17-MER), ... (4 entities in total)
• 機能のキーワード: enterovirus 71, rna-dependent rna polymerase, elongation complex, nucleoside analog, replication
• 由来する生物種: Enterovirus A71; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 70311.82

構造登録者

Zhong, Y.,Gong, P. (登録日: 2024-01-04, 公開日: 2024-12-04, 最終更新日: 2025-01-15)

主引用文献

Jia, X.,Jing, X.,Li, M.,Gao, M.,Zhong, Y.,Li, E.,Liu, Y.,Li, R.,Yao, G.,Liu, Q.,Zhou, M.,Hou, Y.,An, L.,Hong, Y.,Li, S.,Zhang, J.,Wang, W.,Zhang, K.,Gong, P.,Chiu, S.
An adenosine analog shows high antiviral potency against coronavirus and arenavirus mainly through an unusual base pairing mode.
Nat Commun, 15:10750-10750, 2024

PubMed Abstract: By targeting the essential viral RNA-dependent RNA polymerase (RdRP), nucleoside analogs (NAs) have exhibited great potential in antiviral therapy for RNA virus-related diseases. However, most ribose-modified NAs do not present broad-spectrum features, likely due to differences in ribose-RdRP interactions across virus families. Here, we show that HNC-1664, an adenosine analog with modifications both in ribose and base, has broad-spectrum antiviral activity against positive-strand coronaviruses and negative-strand arenaviruses. Importantly, treatment with HNC-1664 demonstrate anti-SARS-CoV-2 efficacy in infected K18-human ACE2 mice, with reduced viral titer and mortality, as well as improved lung injury. Enzymology data demonstrate that HNC-1664 inhibits RNA synthesis mainly at the pre-catalysis stage. The cryo-EM structures of HNC-1664-bound RdRP-RNA complexes from both SARS-CoV-2 and LASV reveal an unusual base pairing mode of HNC-1664 in part due to its base modification, thus revealing its great potency in binding but not catalysis. Under certain circumstances, 1664-TP can be slowly incorporated by RdRP through regular Watson-Crick base pairing, as evidenced by enzymology data and an HNC-1664-incorporated crystal structure of the RdRP-RNA complex. Overall, HNC-1664 achieves broad-spectrum characteristics by favoring an alternative base pairing strategy to non-catalytically block RNA synthesis, providing a novel concept for the rational development of NA drugs.

PubMed: 39737930
DOI: 10.1038/s41467-024-54918-3

実験手法

X-RAY DIFFRACTION (3 Å)