8XO0

Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation

Summary for 8XO0

• Entry DOI: 10.2210/pdb8xo0/pdb
• EMDB information: 38521
• Descriptor: NADH-ubiquinone oxidoreductase chain 3, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial, NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, ... (30 entities in total)
• Functional Keywords: respiratory complex, respiratory supercomplex, electron transport
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 23
• Total formula weight: 574911.58

Authors

Shin, Y.-C.,Liao, M. (deposition date: 2023-12-30, release date: 2024-10-30, Last modification date: 2024-12-04)

Primary citation

Shin, Y.C.,Latorre-Muro, P.,Djurabekova, A.,Zdorevskyi, O.,Bennett, C.F.,Burger, N.,Song, K.,Xu, C.,Paulo, J.A.,Gygi, S.P.,Sharma, V.,Liao, M.,Puigserver, P.
Structural basis of respiratory complex adaptation to cold temperatures.
Cell, 187:6584-, 2024

PubMed Abstract: In response to cold, mammals activate brown fat for respiratory-dependent thermogenesis reliant on the electron transport chain. Yet, the structural basis of respiratory complex adaptation upon cold exposure remains elusive. Herein, we combined thermoregulatory physiology and cryoelectron microscopy (cryo-EM) to study endogenous respiratory supercomplexes from mice exposed to different temperatures. A cold-induced conformation of CI:III (termed type 2) supercomplex was identified with a ∼25° rotation of CIII around its inter-dimer axis, shortening inter-complex Q exchange space, and exhibiting catalytic states that favor electron transfer. Large-scale supercomplex simulations in mitochondrial membranes reveal how lipid-protein arrangements stabilize type 2 complexes to enhance catalytic activity. Together, our cryo-EM studies, multiscale simulations, and biochemical analyses unveil the thermoregulatory mechanisms and dynamics of increased respiratory capacity in brown fat at the structural and energetic level.

PubMed: 39395414
DOI: 10.1016/j.cell.2024.09.029

Experimental method

ELECTRON MICROSCOPY (4.2 Å)