8XKV

Cryo-EM structure of the Ycf2-FtsHi motor complex from Arabidopsis in Apo state

8XKV の概要

• エントリーDOI: 10.2210/pdb8xkv/pdb
• EMDBエントリー: 38425 38426 38427 38428
• 分子名称: Probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic, Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B, UNK, ... (18 entities in total)
• 機能のキーワード: atp-motor, ycf2, ftshi, membrane protein
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 1067463.07

構造登録者

Liang, K.,Zhan, X.,Xu, Q.,Wu, J.,Yan, Z. (登録日: 2023-12-25, 公開日: 2024-09-11, 最終更新日: 2025-06-25)

主引用文献

Liang, K.,Jin, Z.,Zhan, X.,Li, Y.,Xu, Q.,Xie, Y.,Yang, Y.,Wang, S.,Wu, J.,Yan, Z.
Structural insights into the chloroplast protein import in land plants.
Cell, 187:5651-, 2024

PubMed Abstract: Chloroplast proteins are imported via the translocon at the outer chloroplast membrane (TOC)-translocon at the inner chloroplast membrane (TIC) supercomplex, driven by an ATPase motor. The Ycf2-FtsHi complex has been identified as the chloroplast import motor. However, its assembly and cooperation with the TIC complex during preprotein translocation remain unclear. Here, we present the structures of the Ycf2-FtsHi and TIC complexes from Arabidopsis and an ultracomplex formed between them from Pisum. The Ycf2-FtsHi structure reveals a heterohexameric AAA+ ATPase motor module with characteristic features. Four previously uncharacterized components of Ycf2-FtsHi were identified, which aid in complex assembly and anchoring of the motor module at a tilted angle relative to the membrane. When considering the structures of the TIC complex and the TIC-Ycf2-FtsHi ultracomplex together, it becomes evident that the tilted motor module of Ycf2-FtsHi enables its close contact with the TIC complex, thereby facilitating efficient preprotein translocation. Our study provides valuable structural insights into the chloroplast protein import process in land plants.

PubMed: 39197452
DOI: 10.1016/j.cell.2024.08.003

実験手法

ELECTRON MICROSCOPY (3.3 Å)