8XKT

The structure of Spodoptera litura chemosensory protein

Summary for 8XKT

• Entry DOI: 10.2210/pdb8xkt/pdb
• Descriptor: chemosensory protein (2 entities in total)
• Functional Keywords: chemosensory protein, alpha-helical protein, structural protein
• Biological source: Spodoptera litura
• Total number of polymer chains: 1
• Total formula weight: 14415.53

Authors

Xie, W.,Zhang, J.,Jia, Q. (deposition date: 2023-12-24, release date: 2025-01-01, Last modification date: 2026-02-04)

Primary citation

Zhang, J.,Jia, Q.,Xie, W.
Structural plasticity underlies the binding capacities and specificities of chemosensory proteins towards various pesticides.
Pestic Biochem Physiol, 215:106647-106647, 2025

PubMed Abstract: Chemosensory proteins (CSPs) are small proteins that play important roles in insect physiology. Spodoptera litura, a notorious pest, causes considerable losses to the agricultural industry. Recent studies have linked the pesticide resistance of S. litura to CSPs, but the resistance mechanism remains poorly understood due to a dearth of biochemical studies and structural information on the CSP-ligand complexes. In this study, we report the crystal structure of Spodoptera litura CSP3 (SlCSP3). A comparative structural analysis of apo-SlCSP3 with its ligand-bound counterparts or AlphaFold2-predicted CSPs reveals a previously overlooked conformational state with an expanded pocket size. Furthermore, molecular dynamics (MD) simulations highlight the inherent structural plasticity of CSPs. Through virtual screening of potential CSP ligands, numerous agrochemical candidates were identified, with pyrethroids emerging as the primary category. Following docking studies demonstrated that the structurally diverse C-termini of CSPs dictate their binding specificities and affinities. This study offers insights into the structural underpinnings of differential binding propensities and specificities of CSPs, thereby shedding light on their pertinent mechanisms of drug resistance.

PubMed: 41162037
DOI: 10.1016/j.pestbp.2025.106647

Experimental method

X-RAY DIFFRACTION (2.5 Å)