8XI3
Structure of mouse SCMC-14-3-3gama complex
Summary for 8XI3
| Entry DOI | 10.2210/pdb8xi3/pdb |
| EMDB information | 38369 |
| Descriptor | 14-3-3 protein gamma, NACHT, LRR and PYD domains-containing protein 5, Transducin-like enhancer protein 6, ... (4 entities in total) |
| Functional Keywords | complex, oocyte subcortical, phosphorylation, 14-3-3, cytosolic protein |
| Biological source | Mus musculus (house mouse) More |
| Total number of polymer chains | 5 |
| Total formula weight | 258563.47 |
| Authors | |
| Primary citation | Han, Z.,Wang, R.,Chi, P.,Zhang, Z.,Min, L.,Jiao, H.,Ou, G.,Zhou, D.,Qin, D.,Xu, C.,Gao, Z.,Qi, Q.,Li, J.,Lu, Y.,Wang, X.,Chen, J.,Yu, X.,Hu, H.,Li, L.,Deng, D. The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3. Nat Commun, 15:8887-8887, 2024 Cited by PubMed Abstract: The subcortical maternal complex (SCMC) is essential for safeguarding female fertility in mammals. Assembled in oocytes, the SCMC maintains the cleavage of early embryos, but the underlying mechanism remains unclear. Here, we report that 14-3-3, a multifunctional protein, is a component of the SCMC. By resolving the structure of the 14-3-3-containing SCMC, we discover that phosphorylation of TLE6 contributes to the recruitment of 14-3-3. Mechanistically, during maternal-to-embryo transition, the SCMC stabilizes 14-3-3 protein and contributes to the proper control of CDC25B, thus ensuring the activation of the maturation-promoting factor and mitotic entry in mouse zygotes. Notably, the SCMC establishes a conserved molecular link with 14-3-3 and CDC25B in human oocytes/embryos. This study discloses the molecular mechanism through which the SCMC regulates the cell cycle in early embryos and elucidates the function of the SCMC in mammalian early embryogenesis. PubMed: 39406751DOI: 10.1038/s41467-024-53277-3 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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