8X8G

Crystal structure of EndoSz mutant D234M, from Streptococcus equi subsp. Zooepidemicus Sz105, in complex with oligosaccharide G2S2-oxazoline

Summary for 8X8G

• Entry DOI: 10.2210/pdb8x8g/pdb
• Related: 8W4H
• Related PRD ID: PRD_002420
• Descriptor: glycoside hydrolase, N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose, 2-METHYL-4,5-DIHYDRO-(1,2-DIDEOXY-ALPHA-D-GLUCOPYRANOSO)[2,1-D]-1,3-OXAZOLE, ... (5 entities in total)
• Functional Keywords: n-linked glycan, glycoside hydrolase, transglycosylation, hydrolase, g2s2-oxazoline
• Biological source: Streptococcus equi subsp. zooepidemicus Sz105
• Total number of polymer chains: 1
• Total formula weight: 109681.43

Authors

Guan, H.H.,Lin, C.C.,Hsieh, Y.C.,Chen, C.J. (deposition date: 2023-11-27, release date: 2024-07-03, Last modification date: 2024-07-10)

• Primary citation: Hsieh, Y.C.,Guan, H.H.,Lin, C.C.,Huang, T.Y.,Chuankhayan, P.,Chen, N.C.,Wang, N.H.,Hu, P.L.,Tsai, Y.C.,Huang, Y.C.,Yoshimura, M.,Lin, P.J.,Hsieh, Y.H.,Chen, C.J.; Structure-Based High-Efficiency Homogeneous Antibody Platform by Endoglycosidase Sz Provides Insights into Its Transglycosylation Mechanism.; Jacs Au, 4:2130-2150, 2024; PubMed: 38938812; DOI: 10.1021/jacsau.4c00004

Experimental method

X-RAY DIFFRACTION (2.27 Å)