8X8F
Crystal structure of lipoxygenase from Enhygromyxa salina
Summary for 8X8F
| Entry DOI | 10.2210/pdb8x8f/pdb |
| Descriptor | Arachidonate 15-lipoxygenase, CADMIUM ION (3 entities in total) |
| Functional Keywords | 9-lipoxygenase, oxidoreductase |
| Biological source | Enhygromyxa salina |
| Total number of polymer chains | 12 |
| Total formula weight | 852446.57 |
| Authors | |
| Primary citation | Kim, J.W.,Yoon, J.H.,Lee, J.,Cha, H.J.,Seo, P.W.,Lee, T.E.,Bornscheuer, U.T.,Oh, D.K.,Park, J.B.,Kim, J.S. Discovery and Molecular Characterization of a Novel 9 S -Lipoxygenase from Enhygromyxa salina for Fatty Acid Biotransformations. J.Agric.Food Chem., 72:26263-26273, 2024 Cited by PubMed Abstract: Iron-dependent lipoxygenases (LOXs) are involved in the synthesis of oxylipins from polyunsaturated fatty acids. However, they are usually difficult to overexpress in functional form in microbial cell factories. Moreover, 9-LOXs, generating 9-hydroperoxy fatty acids from C18 polyunsaturated fatty acids, have rarely been found from microbial sources. Here, we discovered a novel 9-LOX in the marine myxobacterium (Es-9-LOX). The recombinant enzyme produced in exhibited remarkable activity in the dioxygenation of linoleic acid (LA, ), α-linolenic acid, γ-linolenic acid, and arachidonic acid specifically at the C9 position to yield the product with ()-configuration at catalytic efficiency of 3.94, 1.42, 1.38, and 0.69 μM·s, respectively. The elucidated X-ray crystal structure of Es-9-LOX reveals a long and narrow hydrophobic pocket that allows the substrate to be near the metal ion and the oxygen tunnel. The enzyme was successfully used in a chemoenzymatic reaction to generate a hydroxy fatty acid from LA. Our study thus contributes to the valorization of renewable polyunsaturated fatty acids into a variety of fatty acid derivatives, including hydroxy fatty acids. PubMed: 39536131DOI: 10.1021/acs.jafc.4c01258 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.796 Å) |
Structure validation
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