8X7W

Structure of dimeric human SCMC complex

Summary for 8X7W

• Entry DOI: 10.2210/pdb8x7w/pdb
• EMDB information: 38129
• Descriptor: Maltose/maltodextrin-binding periplasmic protein,NACHT, LRR and PYD domains-containing protein 5, Oocyte-expressed protein homolog, Ubiquitin-like protein SMT3,Transducin-like enhancer protein 6 (3 entities in total)
• Functional Keywords: oocyte, subcortical, complex, cytosolic protein
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 6
• Total formula weight: 499544.55

Authors

Chi, P.,Ou, G.,Liu, S.,Lu, Y.,Li, J.,Li, J.,Wang, X.,Deng, D. (deposition date: 2023-11-26, release date: 2024-10-09, Last modification date: 2024-10-23)

Primary citation

Chi, P.,Ou, G.,Liu, S.,Ma, Q.,Lu, Y.,Li, J.,Li, J.,Qi, Q.,Han, Z.,Zhang, Z.,Liu, Q.,Guo, L.,Chen, J.,Wang, X.,Huang, W.,Li, L.,Deng, D.
Cryo-EM structure of the human subcortical maternal complex and the associated discovery of infertility-associated variants.
Nat.Struct.Mol.Biol., 2024

PubMed Abstract: The functionally conserved subcortical maternal complex (SCMC) is essential for early embryonic development in mammals. Reproductive disorders caused by pathogenic variants in NLRP5, TLE6 and OOEP, three core components of the SCMC, have attracted much attention over the past several years. Evaluating the pathogenicity of a missense variant in the SCMC is limited by the lack of information on its structure, although we recently solved the structure of the mouse SCMC and proposed that reproductive disorders caused by pathogenic variants are related to the destabilization of the SCMC core complex. Here we report the cryogenic electron microscopy structure of the human SCMC and uncover that the pyrin domain of NLRP5 is essential for the stability of SCMC. By combining prediction of SCMC stability and in vitro reconstitution, we provide a method for identifying deleterious variants, and we successfully identify a new pathogenic variant of TLE6 (p.A396T). Thus, on the basis of the structure of the human SCMC, we offer a strategy for the diagnosis of reproductive disorders and the discovery of new infertility-associated variants.

PubMed: 39379527
DOI: 10.1038/s41594-024-01396-2

Experimental method

ELECTRON MICROSCOPY (3.36 Å)