8X7N

Cell-cell adhesion Nanobody/Antigen Pair

8X7N の概要

• エントリーDOI: 10.2210/pdb8x7n/pdb
• 分子名称: Conjugative transfer: aggregate stability, Nb-TraN (2 entities in total)
• 機能のキーワード: bacterial cell adhesion, cell targeting, nanobodies, membrane protein, synthetic biology, antimicrobial protein
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 139049.55

構造登録者

Chen, P.-P.,Hsia, K.-C.,Ting, S.-Y.,Chen, Y.-C. (登録日: 2023-11-24, 公開日: 2024-07-17, 最終更新日: 2026-01-28)

主引用文献

Chen, P.Y.,Chen, Y.C.,Chen, P.P.,Lin, K.T.,Sargsyan, K.,Hsu, C.P.,Wang, W.L.,Hsia, K.C.,Ting, S.Y.
A whole-cell platform for discovering synthetic cell adhesion molecules in bacteria.
Nat Commun, 15:6568-6568, 2024

PubMed Abstract: Developing programmable bacterial cell-cell adhesion is of significant interest due to its versatile applications. Current methods that rely on presenting cell adhesion molecules (CAMs) on bacterial surfaces are limited by the lack of a generalizable strategy to identify such molecules targeting bacterial membrane proteins in their natural states. Here, we introduce a whole-cell screening platform designed to discover CAMs targeting bacterial membrane proteins within a synthetic bacteria-displayed nanobody library. Leveraging the potency of the bacterial type IV secretion system-a contact-dependent DNA delivery nanomachine-we have established a positive feedback mechanism to selectively enrich for bacteria displaying nanobodies that target antigen-expressing cells. Our platform successfully identified functional CAMs capable of recognizing three distinct outer membrane proteins (TraN, OmpA, OmpC), demonstrating its efficacy in CAM discovery. This approach holds promise for engineering bacterial cell-cell adhesion, such as directing the antibacterial activity of programmed inhibitor cells toward target bacteria in mixed populations.

PubMed: 39095377
DOI: 10.1038/s41467-024-51017-1

実験手法

X-RAY DIFFRACTION (3.67 Å)