8WZ9

The crystal structure of Legionella pneumophila adenosylhomocysteinase Lpg2021 in ternary complex with NAD and adenine

Summary for 8WZ9

• Entry DOI: 10.2210/pdb8wz9/pdb
• Descriptor: Adenosylhomocysteinase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ADENINE, ... (5 entities in total)
• Functional Keywords: legionella pneumophila, sah hydrolase, ade, hydrolase
• Biological source: Legionella pneumophila
• Total number of polymer chains: 1
• Total formula weight: 49362.75

Authors

Gao, Y.S.,Xie, R.,Chen, Y.N.,Ma, J.M.,Ge, H.H. (deposition date: 2023-11-01, release date: 2024-11-06, Last modification date: 2024-11-20)

Primary citation

Gao, Y.,Xie, R.,Chen, Y.,Yang, B.,Wang, M.,Hua, L.,Wang, X.,Wang, W.,Wang, N.,Ge, H.,Ma, J.
Structural basis for substrate recognition by a S-adenosylhomocysteine hydrolase Lpg2021 from Legionella pneumophila.
Int.J.Biol.Macromol., 270:132289-132289, 2024

PubMed Abstract: S-Adenosyl-l-homocysteine hydrolase (SAHH) is a crucial enzyme that governs S-adenosyl methionine (SAM)-dependent methylation reactions within cells and regulates the intracellular concentration of SAH. Legionella pneumophila, the causative pathogen of Legionnaires' disease, encodes Lpg2021, which is the first identified dimeric SAHH in bacteria and is a promising target for drug development. Here, we report the structure of Lpg2021 in its ligand-free state and in complexes with adenine (ADE), adenosine (ADO), and 3-Deazaneplanocin A (DZNep). X-ray crystallography, isothermal titration calorimetry (ITC), and molecular docking were used to elucidate the binding mechanisms of Lpg2021 to its substrates and inhibitors. Virtual screening was performed to identify potential Lpg2021 inhibitors. This study contributes a novel perspective to the understanding of SAHH evolution and establishes a structural framework for designing specific inhibitors targeting pathogenic Legionella pneumophila SAHH.

PubMed: 38735607
DOI: 10.1016/j.ijbiomac.2024.132289

Experimental method

X-RAY DIFFRACTION (1.649 Å)