8WO7

Apo state of Arabidopsis AZG1 T440Y

Summary for 8WO7

• Entry DOI: 10.2210/pdb8wo7/pdb
• EMDB information: 37681
• Descriptor: Adenine/guanine permease AZG1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• Functional Keywords: transport protein
• Biological source: Arabidopsis thaliana (thale cress)
• Total number of polymer chains: 2
• Total formula weight: 132346.35

Authors

Xu, L.,Guo, J. (deposition date: 2023-10-06, release date: 2024-01-10, Last modification date: 2024-11-06)

Primary citation

Xu, L.,Jia, W.,Tao, X.,Ye, F.,Zhang, Y.,Ding, Z.J.,Zheng, S.J.,Qiao, S.,Su, N.,Zhang, Y.,Wu, S.,Guo, J.
Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1.
Nat.Plants, 10:180-191, 2024

PubMed Abstract: Cytokinins are essential for plant growth and development, and their tissue distributions are regulated by transmembrane transport. Recent studies have revealed that members of the 'Aza-Guanine Resistant' (AZG) protein family from Arabidopsis thaliana can mediate cytokinin uptake in roots. Here we present 2.7 to 3.3 Å cryo-electron microscopy structures of Arabidopsis AZG1 in the apo state and in complex with its substrates trans-zeatin (tZ), 6-benzyleaminopurine (6-BAP) or kinetin. AZG1 forms a homodimer and each subunit shares a similar topology and domain arrangement with the proteins of the nucleobase/ascorbate transporter (NAT) family. These structures, along with functional analyses, reveal the molecular basis for cytokinin recognition. Comparison of the AZG1 structures determined in inward-facing conformations and predicted by AlphaFold2 in the occluded conformation allowed us to propose that AZG1 may carry cytokinins across the membrane through an elevator mechanism.

PubMed: 38172575
DOI: 10.1038/s41477-023-01590-y

Experimental method

ELECTRON MICROSCOPY (2.9 Å)