8WHA

Structure of DDM1-nucleosome complex in the ADP-BeFx state with DDM1 bound to SHL2 and SHL-2

8WHA の概要

• エントリーDOI: 10.2210/pdb8wha/pdb
• EMDBエントリー: 37537
• 分子名称: Histone H3.1, Histone H4, Histone H2A.6, ... (9 entities in total)
• 機能のキーワード: complex, nucleosome, chromatin remodeling, structural protein-hydrolase-dna complex, gene regulation
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 379211.39

構造登録者

Liu, Y.,Zhang, Z.,Du, J. (登録日: 2023-09-22, 公開日: 2024-04-17)

主引用文献

Liu, Y.,Zhang, Z.,Hu, H.,Chen, W.,Zhang, F.,Wang, Q.,Wang, C.,Yan, K.,Du, J.
Molecular basis of chromatin remodelling by DDM1 involved in plant DNA methylation.
Nat.Plants, 10:374-380, 2024

PubMed Abstract: Eukaryotic gene regulation occurs at the chromatin level, which requires changing the chromatin structure by a group of ATP-dependent DNA translocases-namely, the chromatin remodellers. In plants, chromatin remodellers function in various biological processes and possess both conserved and plant-specific components. DECREASE IN DNA METHYLATION 1 (DDM1) is a plant chromatin remodeller that plays a key role in the maintenance DNA methylation. Here we determined the structures of Arabidopsis DDM1 in complex with nucleosome in ADP-BeF-bound, ADP-bound and nucleotide-free conformations. We show that DDM1 specifically recognizes the H4 tail and nucleosomal DNA. The conformational differences between ADP-BeF-bound, ADP-bound and nucleotide-free DDM1 suggest a chromatin remodelling cycle coupled to ATP binding, hydrolysis and ADP release. This, in turn, triggers conformational changes in the DDM1-bound nucleosomal DNA, which alters the nucleosome structure and promotes DNA sliding. Together, our data reveal the molecular basis of chromatin remodelling by DDM1.

PubMed: 38413824
DOI: 10.1038/s41477-024-01640-z

実験手法

ELECTRON MICROSCOPY (4.05 Å)