8WEJ

Structure of human phagocyte NADPH oxidase in the activated state

8WEJ の概要

• エントリーDOI: 10.2210/pdb8wej/pdb
• EMDBエントリー: 37477
• 分子名称: Cytochrome b-245 light chain, 2-acetamido-2-deoxy-beta-D-glucopyranose, MAGNESIUM ION, ... (15 entities in total)
• 機能のキーワード: nox2, p22, cyba, cybb, tp1170, nox, p67, rac, p47, oxidoreductase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 233881.95

構造登録者

Chen, L.,Liu, X. (登録日: 2023-09-18, 公開日: 2024-01-24, 最終更新日: 2024-10-16)

主引用文献

Liu, X.,Shi, Y.,Liu, R.,Song, K.,Chen, L.
Structure of human phagocyte NADPH oxidase in the activated state.
Nature, 627:189-195, 2024

PubMed Abstract: Phagocyte NADPH oxidase, a protein complex with a core made up of NOX2 and p22 subunits, is responsible for transferring electrons from intracellular NADPH to extracellular oxygen. This process generates superoxide anions that are vital for killing pathogens. The activation of phagocyte NADPH oxidase requires membrane translocation and the binding of several cytosolic factors. However, the exact mechanism by which cytosolic factors bind to and activate NOX2 is not well understood. Here we present the structure of the human NOX2-p22 complex activated by fragments of three cytosolic factors: p47, p67 and Rac1. The structure reveals that the p67-Rac1 complex clamps onto the dehydrogenase domain of NOX2 and induces its contraction, which stabilizes the binding of NADPH and results in a reduction of the distance between the NADPH-binding domain and the flavin adenine dinucleotide (FAD)-binding domain. Furthermore, the dehydrogenase domain docks onto the bottom of the transmembrane domain of NOX2, which reduces the distance between FAD and the inner haem. These structural rearrangements might facilitate the efficient transfer of electrons between the redox centres in NOX2 and lead to the activation of phagocyte NADPH oxidase.

PubMed: 38355798
DOI: 10.1038/s41586-024-07056-1

実験手法

ELECTRON MICROSCOPY (2.79 Å)