8WDT

Crystal structure of the human adenosine A2A receptor in complex with photoresponsive ligand photoNECA(blue)

8WDT の概要

• エントリーDOI: 10.2210/pdb8wdt/pdb
• 分子名称: Adenosine receptor A2a, Antibody Fab fragment light chain, Antibody Fab fragment heavy chain, ... (4 entities in total)
• 機能のキーワード: g-protein coupled receptor, gpcr, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87197.35

構造登録者

Araya, T.,Asada, H.,Iwata, S.,Im, D.H. (登録日: 2023-09-16, 公開日: 2024-01-17, 最終更新日: 2024-11-13)

主引用文献

Araya, T.,Matsuba, Y.,Suzuki, H.,Doura, T.,Nuemket, N.,Nango, E.,Yamamoto, M.,Im, D.,Asada, H.,Kiyonaka, S.,Iwata, S.
Crystal structure reveals the binding mode and selectivity of a photoswitchable ligand for the adenosine A 2A receptor.
Biochem.Biophys.Res.Commun., 695:149393-149393, 2023

PubMed Abstract: Rational synthetic expansion of photoresponsive ligands is important for photopharmacological studies. Adenosine A receptor (AR) is stimulated by adenosine and related in Parkinson's disease and other diseases. Here, we report the crystal structure of the AR in complex with the novel photoresponsive ligand photoNECA (blue) at 3.34 Å resolution. PhotoNECA (blue) was designed for this structural study and the cell-based assay showed a photoresponsive and receptor selective characteristics of photoNECA (blue) for AR. The crystal structure explains the binding mode, photoresponsive mechanism and receptor selectivity of photoNECA (blue). Our study would promote not only the rational design of photoresponsive ligands but also dynamic structural studies of AR.

PubMed: 38171234
DOI: 10.1016/j.bbrc.2023.149393

実験手法

X-RAY DIFFRACTION (3.34 Å)