8WDE

CryoEM structure of the spike protein of human CoV 229E in complex with receptor hAPN (composite map)

8WDE の概要

• エントリーDOI: 10.2210/pdb8wde/pdb
• EMDBエントリー: 37462
• 分子名称: Spike glycoprotein, Aminopeptidase N, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: human aminopeptidase n, viral protein
• 由来する生物種: Human coronavirus 229E; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 607441.33

構造登録者

Hsu, S.T.D.,Tsai, Y.X. (登録日: 2023-09-15, 公開日: 2025-03-05, 最終更新日: 2025-03-12)

主引用文献

Tsai, Y.X.,Chien, Y.C.,Hsu, M.F.,Khoo, K.H.,Hsu, S.D.
Molecular basis of host recognition of human coronavirus 229E.
Nat Commun, 16:2045-2045, 2025

PubMed Abstract: Human coronavirus 229E (HCoV-229E) is the earliest CoV found to infect humans. It binds to the human aminopeptidase N (hAPN) through the receptor binding domain (RBD) of its spike (S) protein to achieve host recognition. We present the cryo-electron microscopy structure of two HCoV-229E S protein in complex with a dimeric hAPN to provide structural insights on how the HCoV-229E S protein opens up its RBD to engage with its host receptor, information that is currently missing among alphacoronaviruses to which HCoV-229E belong. We quantitatively profile the glycosylation of HCoV-229E S protein and hAPN to deduce the glyco-shielding effects pertinent to antigenicity and host recognition. Finally, we present an atomic model of fully glycosylated HCoV-229E S in complex with hAPN anchored on their respective membrane bilayers to recapitulate the structural basis of the first step of host infection by HCoV-229E.

PubMed: 40016196
DOI: 10.1038/s41467-025-57359-8

実験手法

ELECTRON MICROSCOPY (3.6 Å)