8WC9

Cryo-EM structure of the ZH8651-bound mTAAR1-Gq complex

Summary for 8WC9

• Entry DOI: 10.2210/pdb8wc9/pdb
• EMDB information: 37435
• Descriptor: Engineered G-alpha-q subunit, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Trace amine-associated receptor 1, ... (6 entities in total)
• Functional Keywords: zh8651, mtaar1, gq, membrane protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 5
• Total formula weight: 155704.73

Authors

Rong, N.K.,Guo, L.L.,Zhang, M.H.,Li, Q.,Yang, F.,Sun, J.P. (deposition date: 2023-09-11, release date: 2023-12-27, Last modification date: 2024-10-23)

Primary citation

Shang, P.,Rong, N.,Jiang, J.J.,Cheng, J.,Zhang, M.H.,Kang, D.,Qi, L.,Guo, L.,Yang, G.M.,Liu, Q.,Zhou, Z.,Li, X.B.,Zhu, K.K.,Meng, Q.B.,Han, X.,Yan, W.,Kong, Y.,Yang, L.,Wang, X.,Lei, D.,Feng, X.,Liu, X.,Yu, X.,Wang, Y.,Li, Q.,Shao, Z.H.,Yang, F.,Sun, J.P.
Structural and signaling mechanisms of TAAR1 enabled preferential agonist design.
Cell, 186:5347-5362.e24, 2023

PubMed Abstract: Trace amine-associated receptor 1 (TAAR1) senses a spectrum of endogenous amine-containing metabolites (EAMs) to mediate diverse psychological functions and is useful for schizophrenia treatment without the side effects of catalepsy. Here, we systematically profiled the signaling properties of TAAR1 activation and present nine structures of TAAR1-Gs/Gq in complex with EAMs, clinical drugs, and synthetic compounds. These structures not only revealed the primary amine recognition pocket (PARP) harboring the conserved acidic D for conserved amine recognition and "twin" toggle switch for receptor activation but also elucidated that targeting specific residues in the second binding pocket (SBP) allowed modulation of signaling preference. In addition to traditional drug-induced Gs signaling, Gq activation by EAM or synthetic compounds is beneficial to schizophrenia treatment. Our results provided a structural and signaling framework for molecular recognition by TAAR1, which afforded structural templates and signal clues for TAAR1-targeted candidate compounds design.

PubMed: 37963465
DOI: 10.1016/j.cell.2023.10.014

Experimental method

ELECTRON MICROSCOPY (3.2 Å)