8W94

Azumapecten Farreri homopolymeric ferritin (ApF) mutant-H2KE exposed to H2O2 for 2 s

Summary for 8W94

• Entry DOI: 10.2210/pdb8w94/pdb
• Descriptor: Ferritin, FE (III) ION, HYDROGEN PEROXIDE, ... (4 entities in total)
• Functional Keywords: enzyme, metal binding protein
• Biological source: Azumapecten farreri
• Total number of polymer chains: 1
• Total formula weight: 20161.88

Authors

Zhang, T.,Jiao, R. (deposition date: 2023-09-04, release date: 2024-09-11, Last modification date: 2025-03-26)

Primary citation

Jiao, R.,Zhao, G.,Zhang, T.
Structural Insights into the Reaction between Hydrogen Peroxide and Di-iron Complexes at the Ferroxidase Center of Ferritin.
Inorg.Chem., 63:3359-3365, 2024

PubMed Abstract: The Fe(II) oxidation mechanism in the ferroxidase center of heavy chain ferritin has been studied extensively. However, the actual production of HO was found to be substantially lower than expected at low flux of Fe(II) to ferritin subunits. Here, we demonstrated that HO could interact with the di-iron nuclear center, leading to the production of hydroxyl radicals and oxygen. Two reaction intermediates were captured in the ferroxidase center by using the time-lapse crystallographic techniques in a shellfish ferritin. The crystal structures revealed the binding of HO as a μ -1,2-peroxo-diferric species and the binding of O to the diferric structure. This investigation sheds light on the reaction between the di-iron nuclear center and HO and provides insights for the exploitation of metalloenzymes.

PubMed: 38315811
DOI: 10.1021/acs.inorgchem.3c03889

Experimental method

X-RAY DIFFRACTION (2.3 Å)