Summary for 8W1P
| Entry DOI | 10.2210/pdb8w1p/pdb |
| EMDB information | 43729 |
| Descriptor | Cas7, Cas8, Cas5, ... (7 entities in total) |
| Functional Keywords | crispr-cas, genome engineering, rna-guided dna endonuclease, non-coding rna, hnh nuclease, immune system |
| Biological source | Selenomonas sp. More |
| Total number of polymer chains | 12 |
| Total formula weight | 385780.21 |
| Authors | Hirano, S.,Zhang, F. (deposition date: 2024-02-16, release date: 2024-08-07, Last modification date: 2024-09-11) |
| Primary citation | Hirano, S.,Altae-Tran, H.,Kannan, S.,Macrae, R.K.,Zhang, F. Structural determinants of DNA cleavage by a CRISPR HNH-Cascade system. Mol.Cell, 84:3154-, 2024 Cited by PubMed Abstract: Canonical prokaryotic type I CRISPR-Cas adaptive immune systems contain a multicomponent effector complex called Cascade, which degrades large stretches of DNA via Cas3 helicase-nuclease activity. Recently, a highly precise subtype I-F1 CRISPR-Cas system (HNH-Cascade) was found that lacks Cas3, the absence of which is compensated for by the insertion of an HNH endonuclease domain in the Cas8 Cascade component. Here, we describe the cryo-EM structure of Selenomonas sp. HNH-Cascade (SsCascade) in complex with target DNA and characterize its mechanism of action. The Cascade scaffold is complemented by the HNH domain, creating a ring-like structure in which the unwound target DNA is precisely cleaved. This structure visualizes a unique hybrid of two extensible biological systems-Cascade, an evolutionary platform for programmable DNA effectors, and an HNH nuclease, an adaptive domain with a spectrum of enzymatic activity. PubMed: 39111310DOI: 10.1016/j.molcel.2024.07.026 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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