8VTX

Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with macrolone MCX-128, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution

This is a non-PDB format compatible entry.

Summary for 8VTX

• Entry DOI: 10.2210/pdb8vtx/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (64 entities in total)
• Functional Keywords: macrolone; macrolide; fluoroquinolone; erythromycin; ciprofloxacin; antibiotic; 70s ribosome; x-ray structure; inhibition of translation; peptidyl transferase center; nascent peptide exit tunnel; multidrug; resistance; methylation; 23s rrna; a2058; erm, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4571688.86

Authors

Aleksandrova, E.V.,Ma, C.-X.,Klepacki, D.,Alizadeh, F.,Vazquez-Laslop, N.,Liang, J.-H.,Polikanov, Y.S.,Mankin, A.S. (deposition date: 2024-01-27, release date: 2024-08-07)

• Primary citation: Aleksandrova, E.V.,Ma, C.X.,Klepacki, D.,Alizadeh, F.,Vazquez-Laslop, N.,Liang, J.H.,Polikanov, Y.S.,Mankin, A.S.; Macrolones target bacterial ribosomes and DNA gyrase and can evade resistance mechanisms.; Nat.Chem.Biol., 2024; PubMed: 39039256; DOI: 10.1038/s41589-024-01685-3

Experimental method

X-RAY DIFFRACTION (2.4 Å)