8VJ2

Crystal Structure of Macrophage migration inhibitory factor-1 (MIF1) from Onchocerca volvulus

8VJ2 の概要

• エントリーDOI: 10.2210/pdb8vj2/pdb
• 分子名称: Macrophage migration inhibitory factor-1, SODIUM ION (3 entities in total)
• 機能のキーワード: ssgcid, structural genomics, seattle structural genomics center for infectious disease, macrophage migration inhibitory factor-1, cytokine
• 由来する生物種: Onchocerca volvulus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 43398.36

構造登録者

Seattle Structural Genomics Center for Infectious Disease,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2024-01-05, 公開日: 2024-01-17, 最終更新日: 2024-12-18)

主引用文献

Kimble, A.D.,Dawson, O.C.O.,Liu, L.,Subramanian, S.,Cooper, A.,Battaile, K.,Craig, J.,Harmon, E.,Myler, P.,Lovell, S.,Asojo, O.A.
Crystal structure of N-terminally hexahistidine-tagged Onchocerca volvulus macrophage migration inhibitory factor-1.
Acta Crystallogr.,Sect.F, 80:328-334, 2024

PubMed Abstract: Onchocerca volvulus causes blindness, onchocerciasis, skin infections and devastating neurological diseases such as nodding syndrome. New treatments are needed because the currently used drug, ivermectin, is contraindicated in pregnant women and those co-infected with Loa loa. The Seattle Structural Genomics Center for Infectious Disease (SSGCID) produced, crystallized and determined the apo structure of N-terminally hexahistidine-tagged O. volvulus macrophage migration inhibitory factor-1 (His-OvMIF-1). OvMIF-1 is a possible drug target. His-OvMIF-1 has a unique jellyfish-like structure with a prototypical macrophage migration inhibitory factor (MIF) trimer as the `head' and a unique C-terminal `tail'. Deleting the N-terminal tag reveals an OvMIF-1 structure with a larger cavity than that observed in human MIF that can be targeted for drug repurposing and discovery. Removal of the tag will be necessary to determine the actual biological oligomer of OvMIF-1 because size-exclusion chomatographic analysis of His-OvMIF-1 suggests a monomer, while PISA analysis suggests a hexamer stabilized by the unique C-terminal tails.

PubMed: 39503735
DOI: 10.1107/S2053230X24010550

実験手法

X-RAY DIFFRACTION (1.9 Å)