8VFS

oppF ROSE-like RNA thermometer structure

Summary for 8VFS

• Entry DOI: 10.2210/pdb8vfs/pdb
• Descriptor: RNA (27-MER) (2 entities in total)
• Functional Keywords: rna thermometer, regulatory rna, abc transporters, thermoswitch, rna
• Biological source: Providencia stuartii
• Total number of polymer chains: 2
• Total formula weight: 17364.35

Authors

Shaffer, S.N.,Passalacqua, L.F.M.,Abdelsayed, M.M.,Ferre-D'Amare, A.R. (deposition date: 2023-12-21, release date: 2024-12-25, Last modification date: 2025-01-22)

Primary citation

Tong, A.Y.,Tong, E.L.,Hannani, M.A.,Shaffer, S.N.,Santiago, D.,Ferre-D'Amare, A.R.,Passalacqua, L.F.M.,Abdelsayed, M.M.
RNA thermometers are widespread upstream of ABC transporter genes in bacteria.
J.Biol.Chem., 300:107547-107547, 2024

PubMed Abstract: RNA thermometers are temperature-sensing non-coding RNAs that regulate the expression of downstream genes. A well-characterized RNA thermometer motif discovered in bacteria is the ROSE-like element (repression of heat shock gene expression). ATP-binding cassette (ABC) transporters are a superfamily of transmembrane proteins that harness ATP hydrolysis to facilitate the export and import of substrates across cellular membranes. Through structure-guided bioinformatics, we discovered that ROSE-like RNA thermometers are widespread upstream of ABC transporter genes in bacteria. X-ray crystallography, biochemistry, and cellular assays indicate that these RNA thermometers are functional regulatory elements. This study expands the known biological role of RNA thermometers to these key membrane transporters.

PubMed: 38992441
DOI: 10.1016/j.jbc.2024.107547

Experimental method

X-RAY DIFFRACTION (2.7 Å)