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8VAB

Crystal structure of FeII/FeII CtCADD from Chlamydia trachomatis

Summary for 8VAB
Entry DOI10.2210/pdb8vab/pdb
Descriptor4-aminobenzoate synthase, FE (II) ION (3 entities in total)
Functional Keywordsfeii reconstituted form, oxidoreductase
Biological sourceChlamydia trachomatis
Total number of polymer chains1
Total formula weight27880.97
Authors
Phan, H.N.,Swartz, P.D.,Makris, T.M. (deposition date: 2023-12-11, release date: 2024-11-06)
Primary citationPhan, H.N.,Swartz, P.D.,Gangopadhyay, M.,Guo, Y.,Smirnov, A.I.,Makris, T.M.
Assembly of a Heterobimetallic Fe/Mn Cofactor in the para -Aminobenzoate Synthase Chlamydia Protein Associating with Death Domains (CADD) Initiates Long-Range Radical Hole-Hopping.
Biochemistry, 2024
Cited by
PubMed Abstract: Chlamydia protein associating with death domains (CADD) is involved in the biosynthesis of -aminobenzoic acid (pABA) for integration into folate, a critical cofactor that is required for pathogenic survival. CADD activates dioxygen and utilizes its own tyrosine and lysine as synthons to furnish the carboxylate, carbon backbone, and amine group of pABA in a complex multistep mechanism. Unlike other members of the heme oxygenase-like dimetal oxidase (HDO) superfamily that typically house an Fe cofactor, previous activity studies have shown that CADD likely uses a heterobimetallic Fe/Mn center. The structure of the Fe/Mn cofactor and how the conserved HDO scaffold mediates metal selectivity have remained enigmatic. Adopting an metalation approach, CADD was solved in the apo, Fe, Mn, and catalytically active Fe/Mn forms to identify the probable site for Mn binding. The analysis of CADD active-site variants further reinforces the importance of the secondary coordination sphere on cofactor preference for competent pABA formation. Rapid kinetic optical and electron paramagnetic resonance (EPR) studies show that the heterobimetallic cofactor selectively reacts with dioxygen and likely initiates pABA assembly through the formation of a transient tyrosine radical intermediate and a resultant heterobimetallic Mn/Fe cluster.
PubMed: 39471288
DOI: 10.1021/acs.biochem.4c00326
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.65 Å)
Structure validation

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PDB entries from 2024-11-06

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