8V83

60S ribosome biogenesis intermediate (Dbp10 pre-catalytic structure - Overall map)

これはPDB形式変換不可エントリーです。

8V83 の概要

• エントリーDOI: 10.2210/pdb8v83/pdb
• EMDBエントリー: 43017
• 分子名称: Saccharomyces cerevisiae S288C 25S ribosomal RNA, 60S ribosomal protein L6-A, 60S ribosomal protein L7-A, ... (49 entities in total)
• 機能のキーワード: ribosome biogenesis intermediate dead-box atpase, ribosome
• 由来する生物種: Saccharomyces cerevisiae BY4741; 詳細
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 2681053.29

構造登録者

Cruz, V.E.,Weirich, C.S.,Peddada, N.,Erzberger, J.P. (登録日: 2023-12-04, 公開日: 2024-05-01)

主引用文献

Cruz, V.E.,Weirich, C.S.,Peddada, N.,Erzberger, J.P.
The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis.
Nat Commun, 15:3296-3296, 2024

PubMed Abstract: DEAD-box ATPases play crucial roles in guiding rRNA restructuring events during the biogenesis of large (60S) ribosomal subunits, but their precise molecular functions are currently unknown. In this study, we present cryo-EM reconstructions of nucleolar pre-60S intermediates that reveal an unexpected, alternate secondary structure within the nascent peptidyl-transferase-center (PTC). Our analysis of three sequential nucleolar pre-60S intermediates reveals that the DEAD-box ATPase Dbp10/DDX54 remodels this alternate base pairing and enables the formation of the rRNA junction that anchors the mature form of the universally conserved PTC A-loop. Post-catalysis, Dbp10 captures rRNA helix H61, initiating the concerted exchange of biogenesis factors during late nucleolar 60S maturation. Our findings show that Dbp10 activity is essential for the formation of the ribosome active site and reveal how this function is integrated with subsequent assembly steps to drive the biogenesis of the large ribosomal subunit.

PubMed: 38632236
DOI: 10.1038/s41467-024-47616-7

実験手法

ELECTRON MICROSCOPY (2.53 Å)