8UZL

Designed Transmembrane beta-barrel- TMB10_163

8UZL の概要

• エントリーDOI: 10.2210/pdb8uzl/pdb
• 分子名称: Designed Transmembrane beta-barrel TMB10_163, HEXANE-1,6-DIOL (3 entities in total)
• 機能のキーワード: computational design, de novo protein, nanopore, tmb
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 65989.78

構造登録者

Bera, A.K.,Lemma, S.B.,Kang, A.,Baker, D. (登録日: 2023-11-15, 公開日: 2024-07-17, 最終更新日: 2024-07-31)

主引用文献

Berhanu, S.,Majumder, S.,Muntener, T.,Whitehouse, J.,Berner, C.,Bera, A.K.,Kang, A.,Liang, B.,Khan, N.,Sankaran, B.,Tamm, L.K.,Brockwell, D.J.,Hiller, S.,Radford, S.E.,Baker, D.,Vorobieva, A.A.
Sculpting conducting nanopore size and shape through de novo protein design.
Science, 385:282-288, 2024

PubMed Abstract: Transmembrane β-barrels have considerable potential for a broad range of sensing applications. Current engineering approaches for nanopore sensors are limited to naturally occurring channels, which provide suboptimal starting points. By contrast, de novo protein design can in principle create an unlimited number of new nanopores with any desired properties. Here we describe a general approach to designing transmembrane β-barrel pores with different diameters and pore geometries. Nuclear magnetic resonance and crystallographic characterization show that the designs are stably folded with structures resembling those of the design models. The designs have distinct conductances that correlate with their pore diameter, ranging from 110 picosiemens (~0.5 nanometer pore diameter) to 430 picosiemens (~1.1 nanometer pore diameter). Our approach opens the door to the custom design of transmembrane nanopores for sensing and sequencing applications.

PubMed: 39024453
DOI: 10.1126/science.adn3796

実験手法

X-RAY DIFFRACTION (2.5 Å)