8UZA
Cryo-EM structure of GeoCas9 in complex with sgRNA and target DNA
Summary for 8UZA
Entry DOI | 10.2210/pdb8uza/pdb |
EMDB information | 42837 |
Descriptor | CRISPR-associated endonuclease Cas9, sgRNA (107-MER), Target strand DNA, ... (4 entities in total) |
Functional Keywords | rna-guided dna endonuclease, ternary complex, crispr, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Geobacillus stearothermophilus More |
Total number of polymer chains | 4 |
Total formula weight | 202947.24 |
Authors | Eggers, A.R.,Soczek, K.M.,Tuck, O.T.,Doudna, J.A. (deposition date: 2023-11-14, release date: 2024-05-29, Last modification date: 2024-07-03) |
Primary citation | Eggers, A.R.,Chen, K.,Soczek, K.M.,Tuck, O.T.,Doherty, E.E.,Xu, B.,Trinidad, M.I.,Thornton, B.W.,Yoon, P.H.,Doudna, J.A. Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9. Cell, 187:3249-, 2024 Cited by PubMed: 38781968DOI: 10.1016/j.cell.2024.04.031 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.17 Å) |
Structure validation
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