8UZA

Cryo-EM structure of GeoCas9 in complex with sgRNA and target DNA

Summary for 8UZA

Entry DOI	10.2210/pdb8uza/pdb
EMDB information	42837
Descriptor	CRISPR-associated endonuclease Cas9, sgRNA (107-MER), Target strand DNA, ... (4 entities in total)
Functional Keywords	rna-guided dna endonuclease, ternary complex, crispr, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological source	Geobacillus stearothermophilus More
Total number of polymer chains	4
Total formula weight	202947.24
Authors	Eggers, A.R.,Soczek, K.M.,Tuck, O.T.,Doudna, J.A. (deposition date: 2023-11-14, release date: 2024-05-29, Last modification date: 2024-07-03)
Primary citation	Eggers, A.R.,Chen, K.,Soczek, K.M.,Tuck, O.T.,Doherty, E.E.,Xu, B.,Trinidad, M.I.,Thornton, B.W.,Yoon, P.H.,Doudna, J.A. Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9. Cell, 187:3249-, 2024 Cited by PubMed: 38781968 DOI: 10.1016/j.cell.2024.04.031 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.17 Å)

Structure validation