8UQV

Trehalose Synthase (TreS) of Mycobacterium tuberculosis in complex with 6-TreAz compound

8UQV の概要

• エントリーDOI: 10.2210/pdb8uqv/pdb
• EMDBエントリー: 42478
• 分子名称: Trehalose synthase/amylase TreS, alpha-D-glucopyranose, 6-azido-6-deoxy-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: tres, trehalose synthase, mycobacterium tuberculosis, 6-treaz, cytosolic protein
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 264871.96

構造登録者

Pathirage, R.,Ronning, D.R. (登録日: 2023-10-24, 公開日: 2024-03-27, 最終更新日: 2024-04-24)

主引用文献

Kalera, K.,Liu, R.,Lim, J.,Pathirage, R.,Swanson, D.H.,Johnson, U.G.,Stothard, A.I.,Lee, J.J.,Poston, A.W.,Woodruff, P.J.,Ronning, D.R.,Eoh, H.,Swarts, B.M.
Targeting Mycobacterium tuberculosis Persistence through Inhibition of the Trehalose Catalytic Shift.
Acs Infect Dis., 10:1391-1404, 2024

PubMed Abstract: Tuberculosis (TB), caused by (Mtb), is the leading cause of death worldwide by infectious disease. Treatment of Mtb infection requires a six-month course of multiple antibiotics, an extremely challenging regimen necessitated by Mtb's ability to form drug-tolerant persister cells. Mtb persister formation is dependent on the trehalose catalytic shift, a stress-responsive metabolic remodeling mechanism in which the disaccharide trehalose is liberated from cell surface glycolipids and repurposed as an internal carbon source to meet energy and redox demands. Here, using a biofilm-persister model, metabolomics, and cryo-electron microscopy (EM), we found that azidodeoxy- and aminodeoxy-d-trehalose analogues block the Mtb trehalose catalytic shift through inhibition of trehalose synthase TreS (Rv0126), which catalyzes the isomerization of trehalose to maltose. Out of a focused eight-member compound panel constructed by chemoenzymatic synthesis, the natural product 2-trehalosamine exhibited the highest potency and significantly potentiated first- and second-line TB drugs in broth culture and macrophage infection assays. We also report the first structure of TreS bound to a substrate analogue inhibitor, obtained via cryo-EM, which revealed conformational changes likely essential for catalysis and inhibitor binding that can potentially be exploited for future therapeutic development. Our results demonstrate that inhibition of the trehalose catalytic shift is a viable strategy to target Mtb persisters and advance trehalose analogues as tools and potential adjunctive therapeutics for investigating and targeting mycobacterial persistence.

PubMed: 38485491
DOI: 10.1021/acsinfecdis.4c00138

実験手法

ELECTRON MICROSCOPY (3.6 Å)