8UME

Influenza A virus Hemagglutinin H5/Vietnam/1204/2004 in complex with D04 Fab

Summary for 8UME

• Entry DOI: 10.2210/pdb8ume/pdb
• EMDB information: 42377
• Descriptor: Hemagglutinin, Fab Heavy chain, Fab Light chain, ... (4 entities in total)
• Functional Keywords: hemagglutinin, influenza a, antibody, fab, viral protein
• Biological source: Influenza A virus; More
• Total number of polymer chains: 9
• Total formula weight: 333183.54

Authors

Ferreira Ramos, A.S.,Bajic, G. (deposition date: 2023-10-17, release date: 2024-12-25, Last modification date: 2025-02-05)

Primary citation

Feldman, J.,Ramos, A.S.F.,Vu, M.,Maurer, D.P.,Rosado, V.C.,Lingwood, D.,Bajic, G.,Schmidt, A.G.
Human naive B cells recognize prepandemic influenza virus hemagglutinins.
Sci Immunol, 10:eado9572-eado9572, 2025

PubMed Abstract: Understanding the naïve B cell repertoire and its specificity for potential zoonotic threats, such as the highly pathogenic avian influenza (HPAI) H5Nx viruses, may allow prediction of infection- or vaccine-specific responses. However, this naïve repertoire and the possibility to respond to emerging, prepandemic viruses are largely undetermined. Here, we profiled naïve B cell reactivity against a prototypical HPAI H5 hemagglutinin (HA), the major target of antibody responses. We found that the frequency of H5-specific human naïve B cells targeting the HA "head" domain was increased relative to cross-reactive B cells to a circulating seasonal H1N1 strain. We classified the isolated monoclonal antibodies (mAbs) by the HA epitopes engaged and found that selected mAbs neutralized H5N1 at germline. We determined a cryo-electron microscopic structure of one mAb in complex with H5 HA to define its epitope. Our study defines the naïve human B cell repertoire recognizing a potentially zoonotic HPAI.

PubMed: 39854479
DOI: 10.1126/sciimmunol.ado9572

Experimental method

ELECTRON MICROSCOPY (3.1 Å)