8UKC

Solution NMR Structure of the lasso peptide chlorolassin

Summary for 8UKC

• Entry DOI: 10.2210/pdb8ukc/pdb
• NMR Information: BMRB: 31109
• Descriptor: Lassopeptide Chlorolassin (1 entity in total)
• Functional Keywords: lassopeptide, chlorolassin, unknown function
• Biological source: Lentzea jiangxiensis
• Total number of polymer chains: 1
• Total formula weight: 1814.73

Authors

Saad, H.,Zhu, L.,Harris, L.A.,Shelton, K.E.,Mitchell, D.A. (deposition date: 2023-10-12, release date: 2024-09-25, Last modification date: 2024-11-06)

Primary citation

Harris, L.A.,Saad, H.,Shelton, K.E.,Zhu, L.,Guo, X.,Mitchell, D.A.
Tryptophan-Centric Bioinformatics Identifies New Lasso Peptide Modifications.
Biochemistry, 63:865-879, 2024

PubMed Abstract: Lasso peptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) defined by a macrolactam linkage between the N-terminus and the side chain of an internal aspartic acid or glutamic acid residue. Instead of adopting a branched-cyclic conformation, lasso peptides are "threaded", with the C-terminal tail passing through the macrocycle to present a kinetically trapped rotaxane conformation. The availability of enhanced bioinformatics methods has led to a significant increase in the number of secondary modifications found on lasso peptides. To uncover new ancillary modifications in a targeted manner, a bioinformatic strategy was developed to discover lasso peptides with modifications to tryptophan. This effort identified numerous putative lasso peptide biosynthetic gene clusters with core regions of the precursor peptides enriched in tryptophan. Parsing of these tryptophan (Trp)-rich biosynthetic gene clusters uncovered several putative ancillary modifying enzymes, including halogenases and dimethylallyltransferases expected to act upon Trp. Characterization of two gene products yielded a lasso peptide with two 5-Cl-Trp modifications (chlorolassin) and another bearing 5-dimethylallyl-Trp and 2,3-didehydro-Tyr modifications (wygwalassin). Bioinformatic analysis of the requisite halogenase and dimethylallyltransferase revealed numerous other putative Trp-modified lasso peptides that remain uncharacterized. We anticipate that the Trp-centric strategy reported herein may be useful in discovering ancillary modifications for other RiPP classes and, more generally, guide the functional prediction of enzymes that act on specific amino acids.

PubMed: 38498885
DOI: 10.1021/acs.biochem.4c00035

Experimental method

SOLUTION NMR