8UFI

Cryo-EM structure of bovine phosphodiesterase 6

8UFI の概要

• エントリーDOI: 10.2210/pdb8ufi/pdb
• EMDBエントリー: 42208
• 分子名称: Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma, ... (6 entities in total)
• 機能のキーワード: phosphodiesterase, gpcr effector enzyme, signaling protein
• 由来する生物種: Bos taurus (cattle); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 218149.73

構造登録者

Aplin, C.,Cerione, R.A. (登録日: 2023-10-04, 公開日: 2024-01-17, 最終更新日: 2024-02-07)

主引用文献

Aplin, C.,Cerione, R.A.
Probing the mechanism by which the retinal G protein transducin activates its biological effector PDE6.
J.Biol.Chem., 300:105608-105608, 2023

PubMed Abstract: Phototransduction in retinal rods occurs when the G protein-coupled photoreceptor rhodopsin triggers the activation of phosphodiesterase 6 (PDE6) by GTP-bound alpha subunits of the G protein transducin (Gα). Recently, we presented a cryo-EM structure for a complex between two GTP-bound recombinant Gα subunits and native PDE6, that included a bivalent antibody bound to the C-terminal ends of Gα and the inhibitor vardenafil occupying the active sites on the PDEα and PDEβ subunits. We proposed Gα-activated PDE6 by inducing a striking reorientation of the PDEγ subunits away from the catalytic sites. However, questions remained including whether in the absence of the antibody Gα binds to PDE6 in a similar manner as observed when the antibody is present, does Gα activate PDE6 by enabling the substrate cGMP to access the catalytic sites, and how does the lipid membrane enhance PDE6 activation? Here, we demonstrate that 2:1 Gα-PDE6 complexes form with either recombinant or retinal Gα in the absence of the Gα antibody. We show that Gα binding is not necessary for cGMP nor competitive inhibitors to access the active sites; instead, occupancy of the substrate binding sites enables Gα to bind and reposition the PDE6γ subunits to promote catalytic activity. Moreover, we demonstrate by reconstituting Gα-stimulated PDE6 activity in lipid bilayer nanodiscs that the membrane-induced enhancement results from an increase in the apparent binding affinity of Gα for PDE6. These findings provide new insights into how the retinal G protein stimulates rapid catalytic turnover by PDE6 required for dim light vision.

PubMed: 38159849
DOI: 10.1016/j.jbc.2023.105608

実験手法

ELECTRON MICROSCOPY (3.1 Å)