8UDG

S1V2-72 Fab bound to EHA2 from influenza B/Malaysia/2506/2004

Summary for 8UDG

• Entry DOI: 10.2210/pdb8udg/pdb
• EMDB information: 42149
• Descriptor: Hemagglutinin, S1V2-72 heavy chain, S1V2-72 light chain (3 entities in total)
• Functional Keywords: immunoglobulin, complex, hemagglutinin, viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Influenza B virus (B/Malaysia/2506/2004); More
• Total number of polymer chains: 7
• Total formula weight: 145287.81

Authors

Finney, J.,Kong, S.,Walsh Jr, R.M.,Harrison, S.C.,Kelsoe, G. (deposition date: 2023-09-28, release date: 2023-11-15, Last modification date: 2024-10-16)

Primary citation

Finney, J.,Moseman, A.P.,Kong, S.,Watanabe, A.,Song, S.,Walsh Jr., R.M.,Kuraoka, M.,Kotaki, R.,Moseman, E.A.,McCarthy, K.R.,Liao, D.,Liang, X.,Nie, X.,Lavidor, O.,Abbott, R.,Harrison, S.C.,Kelsoe, G.
Protective human antibodies against a conserved epitope in pre- and postfusion influenza hemagglutinin.
Proc.Natl.Acad.Sci.USA, 121:e2316964120-e2316964120, 2024

PubMed Abstract: Phylogenetically and antigenically distinct influenza A and B viruses (IAV and IBV) circulate in human populations, causing widespread morbidity. Antibodies (Abs) that bind epitopes conserved in both IAV and IBV hemagglutinins (HAs) could protect against disease by diverse virus subtypes. Only one reported HA Ab, isolated from a combinatorial display library, protects against both IAV and IBV. Thus, there has been so far no information on the likelihood of finding naturally occurring human Abs that bind HAs of diverse IAV subtypes and IBV lineages. We have now recovered from several unrelated human donors five clonal Abs that bind a conserved epitope preferentially exposed in the postfusion conformation of IAV and IVB HA2. These Abs lack neutralizing activity in vitro but in mice provide strong, IgG subtype-dependent protection against lethal IAV and IBV infections. Strategies to elicit similar Abs routinely might contribute to more effective influenza vaccines.

PubMed: 38147556
DOI: 10.1073/pnas.2316964120

Experimental method

ELECTRON MICROSCOPY (4.98 Å)