8U39

Structure of Human Mitochondrial Chaperonin V72I mutant

8U39 の概要

• エントリーDOI: 10.2210/pdb8u39/pdb
• EMDBエントリー: 41854
• 分子名称: 60 kDa heat shock protein, mitochondrial (1 entity in total)
• 機能のキーワード: chaperonin, human mitochondrial mhsp60, hereditary spastic paraplegia spg13, cryo-em, molecular dynamic simulation, chaperone
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 391126.15

構造登録者

Chen, L.,Wang, J. (登録日: 2023-09-07, 公開日: 2024-08-14)

主引用文献

Syed, A.,Zhai, J.,Guo, B.,Zhao, Y.,Wang, J.C.,Chen, L.
Cryo-EM structure and molecular dynamic simulations explain the enhanced stability and ATP activity of the pathological chaperonin mutant.
Structure, 32:575-584.e3, 2024

PubMed Abstract: Chaperonins Hsp60s are required for cellular vitality by assisting protein folding in an ATP-dependent mechanism. Although conserved, the human mitochondrial mHsp60 exhibits molecular characteristics distinct from the E. coli GroEL, with different conformational assembly and higher subunit association dynamics, suggesting a different mechanism. We previously found that the pathological mutant mHsp60 exhibits enhanced subunit association stability and ATPase activity. To provide structural explanations for the V72I mutational effects, here we determined a cryo-EM structure of mHsp60. Our structural analysis combined with molecular dynamic simulations showed mHsp60 with increased inter-subunit interface, binding free energy, and dissociation force, all contributing to its enhanced subunit association stability. The gate to the nucleotide-binding (NB) site in mHsp60 mimicked the open conformation in the nucleotide-bound state with an additional open channel leading to the NB site, both promoting the mutant's ATPase activity. Our studies highlight the importance of mHsp60's characteristics in its biological function.

PubMed: 38412855
DOI: 10.1016/j.str.2024.02.001

実験手法

ELECTRON MICROSCOPY (3.4 Å)