8U2C

Gaussian mixture model based single particle refinement - ABC transporter (inhibitor-bound ABCG2 from EMPIAR-10374)

8U2C の概要

• エントリーDOI: 10.2210/pdb8u2c/pdb
• 関連するPDBエントリー: 6ETI
• EMDBエントリー: 3953 41845
• 分子名称: Broad substrate specificity ATP-binding cassette transporter ABCG2, 5D3 Fab light chain variable domain, 5D3 Fab heavy chain variable domain, ... (5 entities in total)
• 機能のキーワード: abc transporter, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 331353.39

構造登録者

Chen, M.,Pintilie, G. (登録日: 2023-09-05, 公開日: 2023-11-15, 最終更新日: 2024-10-09)

主引用文献

Chen, M.,Schmid, M.F.,Chiu, W.
Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models.
Nat.Methods, 21:37-40, 2024

PubMed Abstract: Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures.

PubMed: 37973972
DOI: 10.1038/s41592-023-02082-9

実験手法

ELECTRON MICROSCOPY (2.5 Å)