8U28

Gaussian mixture model based single particle refinement - SARS (SARS-CoV-2 Spike Proteins on intact virions from EMPIAR-10492)

Summary for 8U28

Entry DOI	10.2210/pdb8u28/pdb
Related	6zwv
EMDB information	11497 41841
Descriptor	Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
Functional Keywords	coronavirus spike protein, virus
Biological source	Severe acute respiratory syndrome coronavirus 2
Total number of polymer chains	3
Total formula weight	437384.05
Authors	Chen, M.,Pintilie, G. (deposition date: 2023-09-05, release date: 2023-11-15, Last modification date: 2025-05-28)
Primary citation	Chen, M.,Schmid, M.F.,Chiu, W. Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. Nat.Methods, 21:37-40, 2024 Cited by PubMed Abstract: Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures. PubMed: 37973972 DOI: 10.1038/s41592-023-02082-9 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.1 Å)

Structure validation