8U26

Gaussian Mixture Models based single particle refinement - GPCR (Substance P bound to active human neurokinin 1 receptor in complex with miniGs399 from EMPIAR-10786)

Summary for 8U26

• Entry DOI: 10.2210/pdb8u26/pdb
• EMDB information: 24570 41840
• Descriptor: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (6 entities in total)
• Functional Keywords: gpcr, membrane protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 6
• Total formula weight: 141549.56

Authors

Chen, M.,Pintilie, G. (deposition date: 2023-09-05, release date: 2023-11-15, Last modification date: 2024-10-09)

Primary citation

Chen, M.,Schmid, M.F.,Chiu, W.
Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models.
Nat.Methods, 21:37-40, 2024

PubMed Abstract: Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures.

PubMed: 37973972
DOI: 10.1038/s41592-023-02082-9

Experimental method

ELECTRON MICROSCOPY (2.5 Å)