8TZZ
SpG Cas9 with NGC PAM DNA target
Summary for 8TZZ
Entry DOI | 10.2210/pdb8tzz/pdb |
EMDB information | 41775 |
Descriptor | CRISPR-associated endonuclease Cas9/Csn1, RNA (98-MER), DNA (5'-D(P*CP*GP*TP*TP*TP*GP*TP*AP*CP*TP*GP*CP*AP*GP*CP*G)-3'), ... (7 entities in total) |
Functional Keywords | spg-cas9, crispr, cas9, immune system, r-loop, immune system-rna-dna complex, immune system/rna/dna |
Biological source | Streptococcus pyogenes More |
Total number of polymer chains | 5 |
Total formula weight | 206694.03 |
Authors | Bravo, J.P.K.,Hibshman, G.N.,Taylor, D.W. (deposition date: 2023-08-28, release date: 2024-05-01, Last modification date: 2024-05-15) |
Primary citation | Hibshman, G.N.,Bravo, J.P.K.,Hooper, M.M.,Dangerfield, T.L.,Zhang, H.,Finkelstein, I.J.,Johnson, K.A.,Taylor, D.W. Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9. Nat Commun, 15:3663-3663, 2024 Cited by PubMed: 38688943DOI: 10.1038/s41467-024-47830-3 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.56 Å) |
Structure validation
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