8TY6

Disulfide-stabilized HIV-1 CA hexamer in complex with PQBP1 Nt

Summary for 8TY6

• Entry DOI: 10.2210/pdb8ty6/pdb
• EMDB information: 41711
• Descriptor: Capsid protein p24 (1 entity in total)
• Functional Keywords: capsid, innate immune sensor, viral protein
• Biological source: Human immunodeficiency virus 1
• Total number of polymer chains: 6
• Total formula weight: 152767.63

Authors

Piacentini, J.,Pornillos, O.,Ganser-Pornillos, B.K. (deposition date: 2023-08-24, release date: 2024-03-06, Last modification date: 2024-11-06)

Primary citation

Piacentini, J.,Allen, D.S.,Ganser-Pornillos, B.K.,Chanda, S.K.,Yoh, S.M.,Pornillos, O.
Molecular Determinants of PQBP1 Binding to the HIV-1 Capsid Lattice.
J.Mol.Biol., 436:168409-168409, 2024

PubMed Abstract: Human immunodeficiency virus type 1 (HIV-1) stimulates innate immune responses upon infection, including cyclic GMP-AMP synthase (cGAS) signaling that results in type I interferon production. HIV-1-induced activation of cGAS requires the host cell factor polyglutamine binding protein 1 (PQBP1), an intrinsically disordered protein that bridges capsid recognition and cGAS recruitment. However, the molecular details of PQBP1 interactions with the HIV-1 capsid and their functional implications remain poorly understood. Here, we show that PQBP1 binds to HIV-1 capsids through charge complementing contacts between acidic residues in the N-terminal region of PQBP1 and an arginine ring in the central channel of the HIV-1 CA hexamer that makes up the viral capsid. These studies reveal the molecular details of PQBP1's primary interaction with the HIV-1 capsid and suggest that additional elements are likely to contribute to stable capsid binding.

PubMed: 38128824
DOI: 10.1016/j.jmb.2023.168409

Experimental method

ELECTRON MICROSCOPY (3.3 Å)