8TXO

E. coli DNA-directed RNA polymerase transcription elongation complex bound to the unnatural dZ-PTP base pair in the active site

Summary for 8TXO

Entry DOI	10.2210/pdb8txo/pdb
EMDB information	41695
Descriptor	DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
Functional Keywords	complex, unnatural base pairs, synthetic biology, transcription
Biological source	Escherichia coli More
Total number of polymer chains	7
Total formula weight	397457.61
Authors	Aiyer, S.,Shan, Z.,Oh, J.,Wang, D.,Tan, Y.Z.,Lyumkis, D. (deposition date: 2023-08-23, release date: 2024-01-24)
Primary citation	Aiyer, S.,Baldwin, P.R.,Tan, S.M.,Shan, Z.,Oh, J.,Mehrani, A.,Bowman, M.E.,Louie, G.,Passos, D.O.,Dordevic-Marquardt, S.,Mietzsch, M.,Hull, J.A.,Hoshika, S.,Barad, B.A.,Grotjahn, D.A.,McKenna, R.,Agbandje-McKenna, M.,Benner, S.A.,Noel, J.A.P.,Wang, D.,Tan, Y.Z.,Lyumkis, D. Overcoming resolution attenuation during tilted cryo-EM data collection. Nat Commun, 15:389-389, 2024 Cited by PubMed: 38195598 DOI: 10.1038/s41467-023-44555-7 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.1 Å)

Structure validation