8TT7

NMR Assignments and Structure for the Dimeric Kinesin Neck Domain

Summary for 8TT7

• Entry DOI: 10.2210/pdb8tt7/pdb
• NMR Information: BMRB: 52075
• Descriptor: Kinesin heavy chain isoform 5C (1 entity in total)
• Functional Keywords: microtubule motors, intracellular transport, motor protein
• Biological source: Rattus norvegicus (Norway rat)
• Total number of polymer chains: 2
• Total formula weight: 12676.49

Authors

Alexandrescu, A.T. (deposition date: 2023-08-12, release date: 2023-11-01, Last modification date: 2024-05-15)

Primary citation

Seo, D.,Kammerer, R.A.,Alexandrescu, A.T.
Solution NMR assignments and structure for the dimeric kinesin neck domain.
Biomol.Nmr Assign., 17:301-307, 2023

PubMed Abstract: Kinesin is a motor protein, comprised of two heavy and two light chains that transports cargo along the cytoskeletal microtubule filament network. The heavy chain has a neck domain connecting the ATPase motor head responsible for walking along microtubules, with the stalk and subsequent tail domains that bind cargo. The neck domain consists of a coiled coli homodimer with about five heptad repeats, preceded by a linker region that joins to the ATPase head. Here we report H, N, and C NMR assignments and a solution structure for the kinesin neck domain from rat isoform Kif5c. The calculation of the NMR structure of the homodimer was facilitated by unambiguously assigning sidechain NOEs between heptad a and d positions to interchain contacts, since these positions are too far apart to give sidechain contacts in the monomers. The dimeric coiled coil NMR structure is similar to the previously described X-ray structure, whereas the linker region is disordered in solution but contains a short segment with β-strand propensity- the β-linker. Only the coiled coil is protected from solvent exchange, with ∆G values for hydrogen exchange on the order of 4-6 kcal/mol. The high stability of the hydrogen-bonded α-helical structure makes it unlikely that unzippering of the coiled coil is involved in kinesin walking. Rather, the linker region serves as a flexible hinge between the kinesin head and neck.

PubMed: 37861970
DOI: 10.1007/s12104-023-10159-x

Experimental method

SOLUTION NMR