8TPU

Subtomogram averaged consensus structure of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes

これはPDB形式変換不可エントリーです。

8TPU の概要

• エントリーDOI: 10.2210/pdb8tpu/pdb
• EMDBエントリー: 41485
• 分子名称: 40S ribosomal protein S24, 40S ribosomal protein SA, 40S ribosomal protein S3, ... (79 entities in total)
• 機能のキーワード: ribosome, malaria, cryoet, in situ, plasmodium falciparum, translation
• 由来する生物種: Plasmodium falciparum 3D7; 詳細
• タンパク質・核酸の鎖数: 79
• 化学式量合計: 3419265.09

構造登録者

Anton, L.,Cheng, W.,Zhu, X.,Ho, C.M. (登録日: 2023-08-05, 公開日: 2024-08-14, 最終更新日: 2025-12-10)

主引用文献

Anton, L.,Cheng, W.,Haile, M.T.,Dziekan, J.M.,Cobb, D.W.,Zhu, X.,Han, L.,Li, E.,Nair, A.,Lee, C.L.,Wang, H.,Ke, H.,Zhang, G.,Doud, E.H.,Cowman, A.F.,Ho, C.M.
Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug.
Nat.Struct.Mol.Biol., 32:2158-2164, 2025

PubMed Abstract: Our understanding of cellular events is hampered by the gap between the resolution at which we can observe events inside cells and our ability to replicate physiological conditions in test tubes. Here, we show in Plasmodium falciparum, a non-model organism of high medical importance, that this gap can be bridged by using an integrated structural biology approach to visualize events inside the cell at molecular resolution. We determined eight high-resolution structures of the native malarial ribosome in actively translating states inside P. falciparum-infected human erythrocytes using in situ cryo-electron tomography. Following perturbation with a Plasmodium-specific translation inhibitor, we then observed a decrease in elongation factor-bound ribosomal states and an apparent upregulation of ribosome biogenesis in inhibitor-treated parasites. Our work elucidates new molecular details of the malarial translation elongation cycle and demonstrates direct multiscale visualization of drug-induced phenotypic changes in the structure and localization of individual molecules within the native cellular context.

PubMed: 40825879
DOI: 10.1038/s41594-025-01632-3

実験手法

ELECTRON MICROSCOPY (4.1 Å)